Peptide Calculator
Calculate peptide reconstitution, bacteriostatic water volume, and precise syringe draw units with a live visual syringe simulator.
Draw liquid to the 10 unit mark on your U-100 (1 mL) syringe.
Interactive Syringe Visualizer (U-100 • 100 Units)
Common Reconstitution Matrix & Lookup Table
Quick reference for U-100 syringe units across standard peptide vial sizes and BAC water volumes.
| Vial Size | BAC Water Added | Concentration | 100 mcg Dose | 250 mcg Dose | 500 mcg Dose | 1,000 mcg (1mg) |
|---|---|---|---|---|---|---|
| 2 mg | 1.0 mL | 2,000 mcg/mL | 5 Units | 12.5 Units | 25 Units | 50 Units |
| 5 mg | 1.0 mL | 5,000 mcg/mL | 2 Units | 5 Units | 10 Units | 20 Units |
| 5 mg | 2.0 mL | 2,500 mcg/mL | 4 Units | 10 Units | 20 Units | 40 Units |
| 5 mg | 2.5 mL | 2,000 mcg/mL | 5 Units | 12.5 Units | 25 Units | 50 Units |
| 10 mg | 2.0 mL | 5,000 mcg/mL | 2 Units | 5 Units | 10 Units | 20 Units |
| 10 mg | 3.0 mL | 3,333 mcg/mL | 3 Units | 7.5 Units | 15 Units | 30 Units |
| 15 mg | 3.0 mL | 5,000 mcg/mL | 2 Units | 5 Units | 10 Units | 20 Units |
| 20 mg | 2.0 mL | 10,000 mcg/mL | 1 Unit | 2.5 Units | 5 Units | 10 Units |
Step-by-Step Sterile Reconstitution Protocol
Sanitize & Prep
Pop off the protective flip-top caps from both the peptide vial and the Bacteriostatic (BAC) water vial. Swab the rubber stoppers thoroughly with a sterile 70% isopropyl alcohol wipe and allow them to air-dry completely (15–30 seconds).
Draw BAC Water
Using a fresh mixing syringe (e.g. 2mL or 3mL with 21G–25G needle), pull an equal volume of room air into the syringe, insert into the BAC water vial, inject the air to balance pressure, and smoothly draw your required water volume (e.g., 2.0 mL).
Slow Wall Injection
Insert the needle through the peptide vial stopper at a 45° angle aimed at the glass wall. Do not squirt water directly onto the lyophilized powder! Allow the vacuum to gently pull the liquid down the inner wall. Control the plunger speed with your thumb.
Swirl & Refrigerate
NEVER SHAKE THE VIAL! Vigorous agitation denatures protein structures. Gently roll the vial between your palms or swirl in slow circular motions until completely dissolved and crystal clear. Store immediately in a refrigerator at 2°C to 8°C (36°F to 46°F).
Understanding Syringe Unit Mathematics
Insulin syringes are calibrated using standard volumetric definitions where 100 Units equals exactly 1.0 milliliter (1 mL):
Does a 0.5 mL (50-unit) syringe have different units than a 1.0 mL (100-unit) syringe?
No. 10 units on a 0.5 mL syringe is identical to 10 units on a 1.0 mL syringe (exactly 0.10 mL). The crucial distinction is barrel width: smaller barrels spread tick marks wider apart, providing dramatically higher measurement accuracy and reducing dead space.
Storage Guidelines & BAC Water Lifespan
Peptides exist in two distinct physical states with vastly different degradation profiles:
- Lyophilized (Dry Freeze-Dried Powder): Stable at room temperature (under 25°C) for several weeks, or in standard freezer (-20°C) for 12 to 36 months protected from light.
- Reconstituted (Liquid Solution): Must be kept continuously refrigerated at 2°C – 8°C (36°F – 46°F). Do not freeze liquid peptides, as ice crystals can shatter molecular bonds.
- 0.9% Benzyl Alcohol Window: Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial proliferation for up to 28 days after first puncture. Solutions older than 30 days should be retired.
Frequently Asked Questions (FAQ)
- Calculate Solution Concentration: (Vial mg ÷ Water mL) = mg per mL.
- Find Draw Volume in mL: (Desired Dose mg ÷ Concentration mg/mL) = Draw Volume mL.
- Convert to U-100 Units: Draw Volume mL × 100 = Exact Syringe Units.
- 5 mg vial + 2.0 mL water = 10 Units for 250 mcg.
- 5 mg vial + 2.5 mL water = 12.5 Units for 250 mcg.
- 5 mg vial + 1.0 mL water = 5 Units for 250 mcg.
- 10 mg vial + 2.0 mL water = 5 Units for 250 mcg.
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